Minor Wheat Protein Fractions Analysis by Using Capillary Zone Electrophoresis

Minor Wheat Protein Fractions Analysis by Using Capillary Zone Electrophoresis

The wheat proteins soluble in chloroform-methanol mixtures are associated with several kinds of food allergies. A separation method based on capillary zone electrophoresis (CZE) with UV detection was developed for the analysis of these mixtures. An acidic phosphoric acid/β-alanine (pH 2.5) buffer containing HPMC, urea and acetonitrile was used for the separation. The capillary electrophoresis (...

Separation and Characterization of Wheat Protein Fractions by High - Performance Capillary Electrophoresis 1

Cereal Chem. 72(6):527-532 Wheat protein fractions, separated by the Osborne Solvent Fractionaseparated by HPCE. Combined results of this study and our previous tion Procedure, were characterized by high-performance capillary elecstudies provide a catalog of individual gliadin information from HPCE, trophoresis (HPCE). Each fraction was separated on a 27-cm fused-glass HPLC, acid-polyacrylamide...

Determination of protein charge by capillary zone electrophoresis.

The feasibility of employing classical electrophoresis theory to determine the net charge (valence) of proteins by capillary zone electrophoresis is illustrated in this paper. An outline of a procedure to facilitate the interpretation of mobility measurements is demonstrated by its application to a published mobility measurement for Staphylococcal nuclease at pH 8.9 that had been obtained by ca...

Capillary zone electrophoresis pdf

Analysis of illicit amphetamine seizures by capillary zone electrophoresis.

Capillary zone electrophoresis was applied for the determination of amphetamine and related substances in seized drugs. A buffer made of 0.1 M phosphoric acid adjusted to pH 3.0 with triethanolamine was selected. With this background electrolyte, triethanolamine is adsorbed to the capillary wall and the electroosmotic flow is reversed. This gives rise to peaks with good symmetry, high efficienc...